tlr4 inhibitor Search Results


tirap (tlr2 and tlr4) inhibitor peptide set  (Bio-Techne corporation)
94
Bio-Techne corporation tirap (tlr2 and tlr4) inhibitor peptide set
Tirap (Tlr2 And Tlr4) Inhibitor Peptide Set, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TIRAP+(TLR2+and+TLR4)+Inhibitor+Peptide+Set/bio-techne+corporation___nbp2-29331
Average 94 stars, based on 1 article reviews
tirap (tlr2 and tlr4) inhibitor peptide set - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier
tlr2 tlr4 tir domain  (Novus Biologicals)
90
Novus Biologicals tlr2 tlr4 tir domain
Tlr2 Tlr4 Tir Domain, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TIRAP+(TLR2+and+TLR4)+Inhibitor+Peptide+Set/pm30635391-76-20-29
Average 90 stars, based on 1 article reviews
tlr2 tlr4 tir domain - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
peptides  (Novus Biologicals)
93
Novus Biologicals peptides
Peptides, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TLR4+Inhibitor/pmc10412839-81-1-9
Average 93 stars, based on 1 article reviews
peptides - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
tlr2 4 inhibitor  (Novus Biologicals)
92
Novus Biologicals tlr2 4 inhibitor
Tlr2 4 Inhibitor, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TIRAP+(TLR2+and+TLR4)+Inhibitor+Peptide+Set/pm40400028-85-39-45
Average 92 stars, based on 1 article reviews
tlr2 4 inhibitor - by Bioz Stars, 2026-11
92/100 stars
  Buy from Supplier
tlr4 inhibitor  (Novus Biologicals)
93
Novus Biologicals tlr4 inhibitor
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr4 Inhibitor, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TLR4+Inhibitor/pmc08789803-183-43-47
Average 93 stars, based on 1 article reviews
tlr4 inhibitor - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
concentration 10× tirap  (Novus Biologicals)
93
Novus Biologicals concentration 10× tirap
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Concentration 10× Tirap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TIRAP+(TLR2+and+TLR4)+Inhibitor+Peptide+Set/pmc08509240-57-19-29
Average 93 stars, based on 1 article reviews
concentration 10× tirap - by Bioz Stars, 2026-11
93/100 stars
  Buy from Supplier
tlr4 peptide inhibitor set viper  (Novus Biologicals)
91
Novus Biologicals tlr4 peptide inhibitor set viper
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr4 Peptide Inhibitor Set Viper, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TLR4+Inhibitor+Peptide+Set/pmc05748971-155-0-15
Average 91 stars, based on 1 article reviews
tlr4 peptide inhibitor set viper - by Bioz Stars, 2026-11
91/100 stars
  Buy from Supplier
tlr2 tlr4 inhibitor  (Novus Biologicals)
91
Novus Biologicals tlr2 tlr4 inhibitor
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr2 Tlr4 Inhibitor, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TIRAP+(TLR2+and+TLR4)+Inhibitor+Peptide+Set/pmc07604950-93-22-24
Average 91 stars, based on 1 article reviews
tlr2 tlr4 inhibitor - by Bioz Stars, 2026-11
91/100 stars
  Buy from Supplier
tlr4 inhibitor tak-242  (Akaza Research LLC)
90
Akaza Research LLC tlr4 inhibitor tak-242
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr4 Inhibitor Tak 242, supplied by Akaza Research LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/tlr4+inhibitor+tak+242/pmc11887801-41-13-16
Average 90 stars, based on 1 article reviews
tlr4 inhibitor tak-242 - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
tlr4 inhibitor candesartan  (3B Scientific)
90
3B Scientific tlr4 inhibitor candesartan
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr4 Inhibitor Candesartan, supplied by 3B Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/tlr4+inhibitor+candesartan/pmc04461576__14_189_Cao__Suppl-28-16-20
Average 90 stars, based on 1 article reviews
tlr4 inhibitor candesartan - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
tlr4 signaling inhibitor resatorvid  (AbMole Bioscience)
90
AbMole Bioscience tlr4 signaling inhibitor resatorvid
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tlr4 Signaling Inhibitor Resatorvid, supplied by AbMole Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/tlr4+inhibitor+tak+242+m4838/pm37735499-307-6-12
Average 90 stars, based on 1 article reviews
tlr4 signaling inhibitor resatorvid - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier
tak 242  (Bio-Techne corporation)
94
Bio-Techne corporation tak 242
a Co-immunofluorescent staining of <t>TLR4</t> and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and <t>TLR4-deficient</t> ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).
Tak 242, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tlr4+inhibitor/TAK+242/custom%406587%4010%2E1101%2F2025%2E06%2E23%2E661105
Average 94 stars, based on 1 article reviews
tak 242 - by Bioz Stars, 2026-11
94/100 stars
  Buy from Supplier

Image Search Results


a Co-immunofluorescent staining of TLR4 and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and TLR4-deficient ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).

Journal: Communications Biology

Article Title: Intrinsic cardiac adrenergic cells contribute to LPS-induced myocardial dysfunction

doi: 10.1038/s42003-022-03007-6

Figure Lengend Snippet: a Co-immunofluorescent staining of TLR4 and TH in NRVM ICA cell+ , TLR4: toll-like receptor 4, red; TH: ICA cells, green; DAPI: nuclei, blue ( n = 6). b and c NE production as well as TH and DBH expression in rat ICA cells treated with Viper (TLR4 inhibitor, 10 μM) for 2 h prior to LPS for 6 h. ( n = 48, each dot represents one independent repeat). d TLR4 expression in wildtype (WT) and TLR4-deficient ( Tlr4 Lps-del ) NMVM ICA cell+ . e and f NE production and DBH expression in WT and Tlr4 Lps-del NMVM ICA cell+ stimulated with LPS for 6 h, control: saline ( n = 12, each dot represents a biological replicate in d – f ). g Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 36 h after transfection and LPS stimulation. h Luciferase activity of AP-1-luc- and TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with plasmids (0.1 μg/mL) encoding MyD88 and TRIF. i Expression of c-Jun and c-Fos in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA and c-Fos siRNA. j Luciferase activity of TH-promoter-luc- relative to RLTK-luc- in HEK293/hTLR4 cells at 24 h after transfection with MyD88 plasmid, c-Jun siRNA, and c-Fos siRNA, (0.1 μg/mL for plasmid) (each dot represents one independent repeat in ( g – j )). Data are presented as mean ± SEM using one-way ANOVA and Bonferroni multiple comparison test, * P < 0.05, ** P < 0.01, most of the P -values were assigned on the figure. n represents the number of neonatal rats ( a – c ) or neonatal mice ( d – f ).

Article Snippet: α 1 -AR antagonist: Prazosin (Sigma-Aldrich#7791); α 2 -AR antagonist: Yohimbine (Sigma-Aldrich #Y3125); α 2A -AR antagonist: BRL 44408 (Sigma-Aldrich #B4559); β 1 -AR antagonist: CGP20712A (Sigma-Aldrich #C231); β 2 -AR antagonist: ICI-118 551 (Sigma-Aldrich #I127); β 3 -AR antagonist: SR59230A (Sigma-Aldrich #S8688); TLR4 inhibitor: Viper peptide (Novus#NBP2-226244); CaMKII inhibitor: KN-93 Phosphate (Selleckchem #S7423); DBH inhibitor: Nepicastat (MCE MedChem Express#HY-13289); PKA Inhibitor: 14-22 Amide (Calbiochem® #476485).

Techniques: Staining, Expressing, Control, Saline, Luciferase, Activity Assay, Transfection, Plasmid Preparation, Comparison

ICA cell produces no TNF-α due to the elevated Nfkbia and Tnfaip6 expression. Increased NE release by LPS-TLR4 signaling activates β1-AR-CaMKII signaling in cardiomyocytes to regulate NF-κB and mitogen-activated protein kinase pathways, subsequently aggravating myocardial TNF-α production and dysfunction.

Journal: Communications Biology

Article Title: Intrinsic cardiac adrenergic cells contribute to LPS-induced myocardial dysfunction

doi: 10.1038/s42003-022-03007-6

Figure Lengend Snippet: ICA cell produces no TNF-α due to the elevated Nfkbia and Tnfaip6 expression. Increased NE release by LPS-TLR4 signaling activates β1-AR-CaMKII signaling in cardiomyocytes to regulate NF-κB and mitogen-activated protein kinase pathways, subsequently aggravating myocardial TNF-α production and dysfunction.

Article Snippet: α 1 -AR antagonist: Prazosin (Sigma-Aldrich#7791); α 2 -AR antagonist: Yohimbine (Sigma-Aldrich #Y3125); α 2A -AR antagonist: BRL 44408 (Sigma-Aldrich #B4559); β 1 -AR antagonist: CGP20712A (Sigma-Aldrich #C231); β 2 -AR antagonist: ICI-118 551 (Sigma-Aldrich #I127); β 3 -AR antagonist: SR59230A (Sigma-Aldrich #S8688); TLR4 inhibitor: Viper peptide (Novus#NBP2-226244); CaMKII inhibitor: KN-93 Phosphate (Selleckchem #S7423); DBH inhibitor: Nepicastat (MCE MedChem Express#HY-13289); PKA Inhibitor: 14-22 Amide (Calbiochem® #476485).

Techniques: Expressing